Low-Cost Methods for Molecular Characterization of Mutant Plants

Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols

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Éditeur :

Springer


Paru le : 2015-05-08



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Description

This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
Pages
38 pages
Collection
n.c
Parution
2015-05-08
Marque
Springer
EAN papier
9783319162584
EAN PDF
9783319162591

Informations sur l'ebook
Nombre pages copiables
0
Nombre pages imprimables
3
Taille du fichier
1024 Ko
Prix
0,00 €
EAN EPUB
9783319162591

Informations sur l'ebook
Nombre pages copiables
0
Nombre pages imprimables
3
Taille du fichier
584 Ko
Prix
0,00 €

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