Téléchargez le livre : Advanced Photon Counting

Advanced Photon Counting

Applications, Methods, Instrumentation

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Éditeur :

Springer


Collection :

Springer Series on Fluorescence

Paru le : 2015-04-23



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Description

This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.
Pages
370 pages
Collection
Springer Series on Fluorescence
Parution
2015-04-23
Marque
Springer
EAN papier
9783319156354
EAN PDF
9783319156361
Informations sur l'ebook
Nombre pages copiables
3
Nombre pages imprimables
37
Taille du fichier
13363 Ko
Ce livre est accessible aux handicaps Voir les informations sur l'accessibilité
Prix
410,39 €
EAN EPUB
9783319156361
Informations sur l'ebook
Nombre pages copiables
3
Nombre pages imprimables
37
Taille du fichier
6728 Ko
Ce livre est accessible aux handicaps Voir les informations sur l'accessibilité
Prix
410,39 €

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