MicroRNAs as Tools in Biopharmaceutical Production



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Éditeur :

Springer


Paru le : 2012-11-15



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Description

Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells,  miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.
Pages
126 pages
Collection
n.c
Parution
2012-11-15
Marque
Springer
EAN papier
9789400751279
EAN EPUB
9789400751286

Informations sur l'ebook
Nombre pages copiables
1
Nombre pages imprimables
12
Taille du fichier
522 Ko
Prix
94,94 €

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